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Mouse Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) ELISA Kit

ArtNr ABK-KTE70126-96Tx5
Hersteller Abbkine Scientific
Menge 96Tx5
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Mouse Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF17 in samples. An antibody specific for TNFRSF17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Tumor necrosis factor receptor superfamily member 17 microplate
Mouse Tumor necrosis factor receptor superfamily member 17 standard
Mouse Tumor necrosis factor receptor superfamily member 17 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFRSF17. No significant cross-reactivity or interference between Mouse TNFRSF17 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Tumor necrosis factor receptor superfamily member 17 is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b, and to lead to NF-kappaB and MAPK8/JNK activation.
This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.The patient from whose tumor cells the 4; 16 translocation was derived had a chronic intestinal malabsorption syndrome. Histologic and immunohistochemical studies demonstrated a lymphoproliferative syndrome of mature T cells.
Gene ID
21935
Alternative
TNFRSF17; BCM; BCMA; CD269; B cell maturation antigen; B-cell maturation factor
Accession
O88472

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx5
Lieferbar: In stock
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