Mouse Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE70139-48T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Mouse Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit employs a two-site sandwich ELISA to quantitate TMX4 in samples. An antibody specific for TMX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMX4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Thioredoxin-related transmembrane protein 4 microplate
Mouse Thioredoxin-related transmembrane protein 4 standard
Mouse Thioredoxin-related transmembrane protein 4 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMX4. No significant cross-reactivity or interference between Mouse TMX4 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
TMX4, a type I transmembrane protein, was localized to the ER and possessed a Trx-like domain that faced the ER lumen. A maleimide alkylation assay showed that a catalytic CXXC motif in the TMX4 Trx-like domain underwent changes in its redox state depending on cellular redox conditions, and, in the normal state, most of the endogenous TMX4 existed in the oxidized form. The redox potential of this domain indicated that TMX4 could work as a reductase in the environment of the ER. TMX4 had no effect on the acceleration of ER-associated degradation. Because TMX4 interacted with calnexin and ERp57 by co-immunoprecipitation assay, the role of TMX4 may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER.
Gene ID
52837
Alternative
TMX4; DJ971N18.2; KIAA1162; PDIA14; TXNDC13; protein disulfide isomerase family A; member 14; thioredoxin domain containing 13
Accession
Q8C0L0
Menge: 48T
Lieferbar: In stock
Listenpreis: 344,37 €
Preis: 344,37 €
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