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Mouse Protein TMED8 (TMED8) ELISA Kit

ArtNr ABK-KTE70197-96Tx5
Hersteller Abbkine Scientific
Menge 96 T x 5
Quantity options 48 T 96 T 96 T x 5 96 T x 50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias TMED8,FAM15B,MGC126559,family with sequence similarity 15,member B,transmembrane emp24 domain containing 8
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA
Manufacturer - Applications
This Mouse Protein TMED8 (TMED8) ELISA Kit employs a two-site sandwich ELISA to quantitate TMED8 in samples. An antibody specific for TMED8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMED8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMED8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMED8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category
Protein
Manufacturer - Conjugate / Tag
TMED8
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background
TMED8 contains 1 GOLD domain.Members of the p24 (p24/gp25L/emp24/Erp) family of proteins have been shown to be critical components of the coated vesicles that are involved in the transportation of cargo molecules from the endoplasmic reticulum to the Golgi complex. The p24 proteins form hetero-oligomeric complexes and are believed to function as receptors for specific secretory cargo.The identification of the GOLD domain could aid in directed investigation of the role of the p24 proteins in the secretion process. The newly detected group of GOLD-domain proteins, which might simultaneously bind membranes and other proteins, point to the existence of a novel class of adaptors that could have a role in the assembly of membrane-associated complexes or in regulating assembly of cargo into membranous vesicles.
Description
This Mouse Protein TMED8 (TMED8) ELISA Kit employs a two-site sandwich ELISA to quantitate TMED8.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Mouse Protein TMED8 (TMED8) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMED8. No significant cross-reactivity or interference between Mouse TMED8 and analogues was observed.
Usage Notes
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components
• Mouse Protein TMED8 microplate
• Mouse Protein TMED8 standard
• Mouse Protein TMED8 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Limit of detection
Please inquire
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type
Sandwich ELISA (quantitative)
Calibration range
Please inquire
Detection method
Colorimetric
Sample type
Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment
ELISA Testing
Manufacturer - Search Terms
TMED8, FAM15B, MGC126559, family with sequence similarity 15, member B, transmembrane emp24 domain containing 8

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 T x 5
Lieferbar: In stock
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