Mouse Spectrin alpha chain, brain (SPTAN1) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Spectrin alpha chain, brain microplate
Mouse Spectrin alpha chain, brain standard
Mouse Spectrin alpha chain, brain detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Alpha-Fodrin is an intracellular, actin-binding, organ-specific protein of the cytoskeleton. The network of actin and fodrin situated below the plasma membrane of secretorial cells, is important for the alignment of secretorial vesicles to the plasma membrane during secretorial processes. During apoptosis the alpha-fodrin dimer is cleaved into a 120 kDa breakdown product, which is found abundantly in the salivary gland. This proteolysis of fodrin may be a consequence of protease activation during apoptosis. The cleavage product of 120 kDa alpha-fodrin was found to be an important autoantigen in the pathogenesis of organ-specific autoimmune esponse. Clinical studies have shown, that in patients with Sjögren Syndrome alpha-fodrin is involved in the stimulation of peripheral blood T-cells.

P16546