Mouse Synaptotagmin-1 (SYT1) ELISA Kit

This Mouse Synaptotagmin-1 (SYT1) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT1 in samples. An antibody specific for SYT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

SYT1

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Mouse Synaptotagmin-1 (SYT1) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT1.

Mouse Synaptotagmin-1 (SYT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYT1. No significant cross-reactivity or interference between Mouse SYT1 and analogues was observed.

• Mouse Synaptotagmin-1 microplate
• Mouse Synaptotagmin-1 standard
• Mouse Synaptotagmin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Please inquire

Cell culture supernatants, Serum, Plasma, Other biological fluids

SYT1, DKFZp781D2042, P65, SVP65, SYT, synaptotagmin 1