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Mouse Antigen peptide transporter 1 (TAP1) ELISA Kit

ArtNr ABK-KTE70340-96T
Hersteller Abbkine Scientific
Menge 96T
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Mouse Antigen peptide transporter 1 (TAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAP1 in samples. An antibody specific for TAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Antigen peptide transporter 1 microplate
Mouse Antigen peptide transporter 1 standard
Mouse Antigen peptide transporter 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Antigen peptide transporter 1 (TAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAP1. No significant cross-reactivity or interference between Mouse TAP1 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
The membrane-associated protein encoded by Tap1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This protein forms a heterodimer with Tap2 that transports short peptides from the cytosol into the endoplasmic reticulum lumen. Mutations in the human gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for Tap1.
Gene ID
21354
Alternative
TAP1; DAAP-57C1.5; ABC17; ABCB2; APT1; D6S114E; FLJ26666; FLJ41500; PSF1; RING4; TAP1*0102N; TAP1N; ABC transporter; MHC 1; ATP-binding cassette; sub-family B (MDR/TAP); member 2; antigen peptide transporte
Accession
P21958

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96T
Lieferbar: In stock
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Lieferung vsl. bis 18.09.2025 

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