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Mouse Matrix metalloproteinase 9 (MMP9) ELISA Kit

Mouse Matrix metalloproteinase 9 (MMP9) ELISA Kit

ArtNr	ABK-KTE70996-96Tx5
Hersteller	Abbkine Scientific
Menge	96 T x 5
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	ELISA Cardiovascular Research Atherosclerosis ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	MMP9,GELB,Gelatinase B,CLG4B,CLG4-B,92 KDa Gelatinase,92kDa Type IV Collagenase,MANDP2,macrophage gelatinase,matrix metalloproteinase 9,type V collagenase
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA
Manufacturer - Applications	This Mouse Matrix metalloproteinase 9 (MMP9) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP9 in samples. An antibody specific for MMP9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	MMP9
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	Mmp9 encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades collagens of type IV, V and XI, and elastin. Mice lacking the encoded protein exhibit an abnormal pattern of skeletal growth plate vascularization and ossification, reduced keratinocyte hyperproliferation at all neoplastic stages, a decreased incidence of invasive tumors, and resistance to experimental autoimmune encephalomyelitis.
Description	This Mouse Matrix metalloproteinase 9 (MMP9) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP9.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Mouse Matrix metalloproteinase 9 (MMP9) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MMP9. No significant cross-reactivity or interference between Mouse MMP9 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Mouse Matrix metalloproteinase 9 microplate • Mouse Matrix metalloproteinase 9 standard • Mouse Matrix metalloproteinase 9 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	MMP9, GELB, Gelatinase B, CLG4B, CLG4-B, 92 KDa Gelatinase, 92kDa Type IV Collagenase, MANDP2, macrophage gelatinase, matrix metalloproteinase 9, type V collagenase

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 96 T x 5

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Lieferung vsl. bis 04.12.2025

Express-Lieferung: 17 Tage sparen

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