Mouse Hepatocyte growth factor activator (HGFAC) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Hepatocyte growth factor activator microplate
Mouse Hepatocyte growth factor activator standard
Mouse Hepatocyte growth factor activator detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

HGFAC belongs to peptidase family S1. It is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Miyazawa et al. (1993) determined the partial amino acid sequence of the homologous human serine protease and used it to design degenerate primers to screen a human liver cDNA library. They identified a 655-amino acid inactive precursor protein, designated hepatocyte growth factor activator (HGFAC), with a calculated molecular mass of 70, 681 Da. They determined that the active protein purified from serum is derived from the C terminus of the precursor by proteolytic cleavage of the bonds between arg372-val373 and arg407-ile408.

HGFAC; RP11-529E10.2; HGFA; MGC138395; MGC138397;