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Rat Tryptophan Hydroxylase (TPH) ELISA Kit

Rat Tryptophan Hydroxylase (TPH) ELISA Kit

ArtNr	ABK-KTE100042-96Tx50
Hersteller	Abbkine Scientific
Menge	96 T x 50
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Neuroscience Neural Signaling ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Rat (Rattus norvegicus)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	TPH1,MGC119994,TPRH,TRPH,L-tryptophan hydroxylase,indoleacetic acid-5-hydroxylase,tryptophan hydroxylase (tryptophan 5-monooxygenase)
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA
Manufacturer - Applications	This Rat Tryptophan Hydroxylase (TPH) ELISA Kit employs a two-site sandwich ELISA to quantitate TPH1 in samples. An antibody specific for TPH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	TPH1
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	TPH2 encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by the mouse ortholog.
Description	This Rat Tryptophan Hydroxylase (TPH) ELISA Kit employs a two-site sandwich ELISA to quantitate TPH1.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Rat Tryptophan Hydroxylase (TPH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TPH1. No significant cross-reactivity or interference between Rat TPH1 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Rat Tryptophan Hydroxylase microplate • Rat Tryptophan Hydroxylase standard • Rat Tryptophan Hydroxylase detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	TPH1, MGC119994, TPRH, TRPH, L-tryptophan hydroxylase, indoleacetic acid-5-hydroxylase, tryptophan hydroxylase (tryptophan 5-monooxygenase)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 96 T x 50

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Lieferung vsl. bis 04.12.2025

Express-Lieferung: 13 Tage sparen

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