Hersteller |
Abbkine Scientific
|
Kategorie |
|
Typ |
Elisa-Kit |
Specific against |
Rat |
Applikationen |
ELISA |
Menge |
48T |
ArtNr |
ABK-KTE100701-48T |
eClass 6.1 |
32160605 |
eClass 9.0 |
32160605 |
Lieferbar |
|
Applications notes |
This Rat Galectin-4 (LGALS4) ELISA Kit employs a two-site sandwich ELISA to quantitate LGALS4 in samples. An antibody specific for LGALS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method |
Colorimetric |
Sample type |
Cell culture supernatants#Serum#Plasma#Other biological fluids |
Assay type |
Sandwich ELISA (quantitative) |
Assay duration |
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Kit components |
Rat Galectin-4 microplate Rat Galectin-4 standard Rat Galectin-4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers |
Features & Benefits |
Rat Galectin-4 (LGALS4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LGALS4. No significant cross-reactivity or interference between Rat LGALS4 and analogues was observed. |
Usage notes |
Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate. |
Storage instructions |
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
Galectin-4 belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain. The two domains have all the conserved galectin signature amino acids, but their overall sequences are only approximately 40% identical. Both domains bind lactose with a similar affinity as other galectins, but their respective preferences for other disaccharides, and larger saccharides, are distinctly different. Thus galectin-4 has a property of a natural cross-linker, but in a modified sense since each domain prefers a different subset of ligands. Similarly to other galectins, galectin-4 is synthesized as a cytosolic protein, but can be externalized. During development and in adult normal tissues, galectin-4 is expressed only in the alimentary tract, from the tongue to the large intestine. |
Gene ID |
25474 |
Alternative |
LGALS4; GAL4; L36LBP; L-36 lactose-binding protein; galectin 4; galectin-4; lactose-binding lectin 4 |
Accession |
P38552 |
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