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Rat Kinesin-1 heavy chain (KIF5B) ELISA Kit

Rat Kinesin-1 heavy chain (KIF5B) ELISA Kit

ArtNr	ABK-KTE100726-96Tx50
Hersteller	Abbkine Scientific
Menge	96 T x 50
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Oncology Neuroscience Neural Lineage Markers By Cell Type Carcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Rat (Rattus norvegicus)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	KIF5B,KINH,KNS,KNS1,UKHC,kinesin 1 (110-120kD),kinesin heavy chain
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Manufacturer - Applications	This Rat Kinesin-1 heavy chain (KIF5B) ELISA Kit employs a two-site sandwich ELISA to quantitate KIF5B in samples. An antibody specific for KIF5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIF5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIF5B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF5B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background	Kinesins are microtubule-based motor proteins involved in the transport of organelles in eukaryotic cells. They The predicted 963-amino acid protein has 63% sequence identity to the Drosophila KHC. Immunoblot analysis using antibodies against squid KHC detected a 120-kD protein in CV-1 monkey kidney epithelial cells. Immunoblot analysis using antibodies against squid KHC detected a 120-kD protein in CV-1 monkey kidney epithelial cells. Immunofluorescence studies showed that KNS1 expressed in CV-1 cells had both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules but not vimentin (VIM) intermediate filaments; the KNS1 N- and C-terminal domains, but not the alpha-helical rod domain, also colocalized with microtubules.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Rat Kinesin-1 heavy chain (KIF5B) ELISA Kit has high sensitivity and excellent specificity for detection of Rat KIF5B. No significant cross-reactivity or interference between Rat KIF5B and analogues was observed.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Rat Kinesin-1 heavy chain microplate Rat Kinesin-1 heavy chain standard Rat Kinesin-1 heavy chain detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 96 T x 50

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Lieferung vsl. bis 01.01.2026

Express-Lieferung: 7 Tage sparen

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