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Rat Calbindin (CALB1) ELISA Kit

Rat Calbindin (CALB1) ELISA Kit

ArtNr	ABK-KTE100848-96Tx50
Hersteller	Abbkine Scientific
Menge	96 T x 50
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience ELISA ELISA & Immunoassays ELISA Kits Immunoassay Calcium Binding Protein Markers Neurodegeneration Markers
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Rat (Rattus norvegicus)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CALB1,CALB,RTVL-H protein,calbindin 1,calbindin 1,(28kD)
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Manufacturer - Applications	This Rat Calbindin (CALB1) ELISA Kit employs a two-site sandwich ELISA to quantitate CALB1 in samples. An antibody specific for CALB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCALB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CALB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CALB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background	Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. The sequence showed an open reading frame coding for a protein of 261 amino acids, containing 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The preliminary data suggested that the 29-kD protein in brain is encoded by a different gene.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Rat Calbindin (CALB1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CALB1. No significant cross-reactivity or interference between Rat CALB1 and analogues was observed.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Rat Calbindin microplate Rat Calbindin standard Rat Calbindin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 96 T x 50

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Lieferung vsl. bis 01.01.2026

Express-Lieferung: 7 Tage sparen

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