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Rat Growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin) ELISA Kit

Rat Growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin) ELISA Kit

ArtNr	ABK-KTE100970-48T
Hersteller	Abbkine Scientific
Menge	48 T
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	Neuroscience Metabolism Diabetes Glucose Homeostasis Regulation of Appetite Molecular Targets for Neuroscience Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Rat (Rattus norvegicus)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	GHRP-Ghrelin
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Manufacturer - Applications	This Rat Growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin) ELISA Kit employs a two-site sandwich ELISA to quantitate GHRP-Ghrelin in samples. An antibody specific for GHRP-Ghrelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGHRP-Ghrelin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GHRP-Ghrelin is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GHRP-Ghrelin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background	Growth hormone secretagogue receptor is a G protein-coupled receptor which binds ghrelin and plays a role in energy homeostasis and regulation of body weight.Ghrelin is an appetite-regulating factor secreted from peripheral organs which is involved in regulation of energy homoeostasis via binding to the receptor resulting in the secretion of growth hormone by the pituitary gland. The pathway activated by binding of Ghrelin to the Growth hormone secretagogue receptor, GHSR1a, regulates the activation of the downstream mitogen-activated protein kinase, Akt, nitric oxide synthase, and AMPK cascades in different cellular systems. One of the important features of GHSR1a displays constitutive activity possessing basal activity in the absence of an agonist, resulting in a high degree of receptor internalization as well as of signaling activity.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Rat Growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin) ELISA Kit has high sensitivity and excellent specificity for detection of Rat GHRP-Ghrelin. No significant cross-reactivity or interference between Rat GHRP-Ghrelin and analogues was observed.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Rat Growth hormone releasing peptide-Ghrelin microplate Rat Growth hormone releasing peptide-Ghrelin standard Rat Growth hormone releasing peptide-Ghrelin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 48 T

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Lieferung vsl. bis 01.01.2026

Express-Lieferung: 7 Tage sparen

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