Rabbit Cytosolic phospholipase A2 (PLA2G4A) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Rabbit Cytosolic phospholipase A2 microplate
Rabbit Cytosolic phospholipase A2 standard
Rabbit Cytosolic phospholipase A2 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Cytosolic phospholipases A2 (cPLA2): The intracellular PLA2 are also Ca-dependent, but they have completely different 3D structure and significantly larger than secreted PLA2 (more than 700 residues). They include C2 domain and large catalytic domain. These phospholipases are involved in cell signaling processes, such as inflammatory response. The produced Arachidonic acid is both a signaling molecule and the precursor for other signalling molecules termed eicosanoids. These include leukotrienes and prostaglandins. Some eicosanoids are synthesized from diacylglycerol, released from the lipid bilayer by phospholipase C (see below). Phospholipases A2 can be classified based on sequence homology.PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands

PLA2G4A; MGC126350; PLA2G4; cPLA2-alpha; calcium-dependent phospholipid-binding protein; cytosolic phospholipase A2; group IVA; lysophospholipase; phosphatidylcholine 2-acylhydrolase