LDH Cytotoxicity Assay Kit

Abbkine LDH Cytotoxicity Assay Kit provides a simple and easy colorimetric assay for the study of cytotoxicity. This assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the generated color correlates directly with the cell number lysed. In a typical cytotoxicity assay, target cells are cultured with a cytotoxic chemical agent or a cytotoxic cell (NK cell, cytotoxic T cells) to induce target cell death and LDH release. The LDH-containing supernatants are transferred to wells of a new 96-well assay plate and mixed with the LDH Reaction solution. After an incubation of 30 minutes at room temperature, the absorbance at 565nm (A565) is read using a plate reader.

Abbkine LDH Cytotoxicity Assay Kit provides a simple and easy colorimetric assay for the study of cytotoxicity.

- Assay Buffer
- Lactic Acid Solution
- MTT Solution
- PES Solution
- LDH Positive Control
- NAD+ Solution
- Triton X-100 (10%)

Store at -20C and Keep in dark. The Kit has a storage time of 6 months from receipt.

Performing an initial titration experiment to determine the optimal number of cells per well of the target cell you plan to use.