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CheKine™ Micro Coenzyme II NADP(H) Assay Kit

ArtNr ABK-KTB1010-96T
Hersteller Abbkine Scientific
Menge 96 T
Quantity options 48 T 96 T
Kategorie
Typ Assay-Kit
Specific against other
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Cell metabolism detection kit
Manufacturer - Applications
Abbkine CheKine NADP/NADPH Assay Kit provides a convenient tool for sensitive detection of the NADP and NADPH, and their ratio in various tissues and subcellular organelles. This Kit is based on enzyme cycling reaction (It does not recognize NAD+/NADH), in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at OD565 nm, is proportionate to the NADP+ /NADPH concentration in the sample.
Manufacturer - Category
Cell analysis
Manufacturer - Conjugate / Tag
NADP/NADPH; NADP; NADPH
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Background
Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the pentose phosphate pathway (PPP) is the major source of NADPH produced in animal cells. There is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissues.
Description
Abbkine CheKine™ Micro Coenzyme II NADP(H) Assay Kit is designed to detect the NADP and NADPH, and their ratio in various tissues and cell.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
• Detect NADP+/NADPH concentrations and ratios in cell or tissue extracts.
• Detection limit of 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.
Usage Notes
• Addition of Working Reagent should be quick and mixing should be brief but thorough.
• The following substances interfere and should be avoided in sample preparation. EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%).
Kit components
• Assay Buffer
• Glucose (1 M)
• WST-8 Solution
• Enhancer Solution
• NADP Cycling Enzyme Mix
• NADPH standard (10 mM)
• NADP Extraction Buffer
• NADPH Extraction Buffer
Calibration range
0.5 µM-10 µM

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 T
Lieferbar: In stock
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