CheKine™ Micro Catalase (CAT) Activity Assay Kit

Cell metabolism detection kit

Cell analysis

Gel pack with blue ice.

Catalase (EC 1.11.1.6), is a common antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen, ubiquitously present in aerobic cells containing a cytochrome system. Hydrogen peroxide is highly deleterious to the cell and its accumulation will cause oxidation of cellular targets such as DNA, proteins, and lipids, thus leading to mutagenesis and cell death. Removal of the hydrogen peroxide (H2O2) from cells by using catalase provides protection against oxidative damage to the cell. The role of catalase in oxidative stress related diseases has been widely studied. Catalase also demonstrates peroxidatic activity, in which low molecular weight alcohols can serve as electron donors. Aliphatic alcohols are specific substrates for catalase, however, other enzymes with peroxidatic activity do not utilize these substrates.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• If not assayed immediately, samples can be stored at -80°C.
• Overheating can inactivate catalse. The enzyme should be kept cold during sample preparation and assaying.
• In general, catalase is very unstable at high dilution. It is recommned to store samples concentrated and assay within 30 minutes afrer dilution.