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CheKine™ Micro Lactate Dehydrogenase (LDH) Assay Kit

ArtNr ABK-KTB1110-96T
Hersteller Abbkine Scientific
Menge 96 T
Quantity options 480 T 48 T 96 T
Kategorie
Typ Assay-Kit
Specific against other
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Cell metabolism detection kit
Manufacturer - Applications
CheKine Lactate Dehydrogenase Assay Kit provides a simple and easy colorimetric assay for measuring Lactate Dehydrogenase in serum, plasma, cell culture supernatants, tissue/cell lysates culture medium, fermentation and other biological fluids. In this colorimetric assay, LDH reduces NAD to NADH, which then interacts with a probe to produce a color (?max = 450 nm). The assay is quick and convenient.
Manufacturer - Category
Cell analysis
Manufacturer - Conjugate / Tag
LDH
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
Storage at –20°C and Keep from light. Stable for at least 6 months at recommended temperature from date of shipment.
Background
Lactate dehydrogenase (LDH) is an oxidoreductase (EC 1.1.1.27) presents in a wide variety of organisms. It catalyses the inter-conversion of pyruvate and lactate with concomitant inter-conversion of NADH and NAD+. It converts pyruvate, the final product of glycolysis, to lactate in hypoxic conditions. LDH quantification is of clinical interest as serum levels of certain LDH isozymes reflect pathological conditions in particular tissues. When disease or injury or toxic material damages tissues, the cells LDH is released into the bloodstream. Since LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells.
Description
CheKine™ Lactate Dehydrogenase Assay Kit provides a simple and easy colorimetric assay for measuring LDH in samples.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
• Optimized procedure for measuring Lactate Dehydrogenase (LDH) in various samples containing serum, plasma, cell culture supernatants, tissue/cell lysates culture medium, plant tissues, fermentation and other biological fluids.
• Simple and easy colorimetric assay.
• Quick and convenient.
Usage Notes
• Briefly centrifuge small vials at low speed prior to opening.
• Performing several dilutions of your sample to ensure the readings are within the standard value range.
• Use fresh samples. If not assayed immediately, samples can be stored at -80°C for one month.
Kit components
• Assay Buffer
• Lactate
• NAD
• WST-8
• Enhancer
• Lactate Dehydrogenase Standard (100 U/mL)
Calibration range
1- 20 U/mL

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 T
Lieferbar: In stock
lieferbar

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