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ExKine™ Membrane and Cytoplasmic Protein Extraction kit

ArtNr ABK-KTP3005-50T
Hersteller Abbkine Scientific
Menge 50 T
Quantity options 200 T 50 T
Kategorie
Typ Assay-Kit
Specific against other
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Alias Null
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Organelle extraction and staining
Manufacturer - Applications
Abbkine ExKine Membrane and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of non-denatured, active cytoplasmic and membrane protein from mammalian cultured cells or tissues, which can be used directly in a variety of proteomics application. The cells are first broken by homogenization to allow the release of cytosolic and membrane proteins, after which membrane proteins are obtained by high speed centrifugation. Extraction efficiencies and yields will vary depending on cell type as well as the number of times the integral membrane protein spans the lipid bilayer.
Manufacturer - Category
Cell analysis
Manufacturer - Conjugate / Tag
Cytoplasmic
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
Background
The preparation of an extract from membrane is often the first step in studying membrane proteins and their interactions. The resulting preparation can be used directly in Western blotting, Dot blot, Immunoprecipitation, or as a starting point for the purification of specific proteins. The preparation of an extract from Cytoplasm is often the first step in studying Cytoplasmic proteins and their interactions. The resulting preparation can be used directly in Western blotting, Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.
Description
Abbkine ExKine™ Membrane and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of cytoplasmic and membrane protein.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
• Versatile—suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations.

• Fast and convenient—non-denatured, active proteins are purified in less than two hours.

• Compatible—apply in various downstream assays, including Western blotting, protein assays, enzyme activity assays, etc.
Usage Notes
Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Kit components
• Cytoplasmic Extraction Buffer (CEB) (5x)
• Membrane Extraction Buffer (MEB)
• Protease Inhibitor (100x)
Product Assortment
WB, IP
Manufacturer - Search Terms
Membrane, Cytoplasmic, Membrane Protein, Cytoplasmic Protein

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 T
Lieferbar: In stock
lieferbar

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