Vergleich

ExKine™ Nuclei Extraction Kit (High Purity)

ArtNr ABK-KTP4002-20T
Hersteller Abbkine Scientific
Menge 20 T
Quantity options 100 T 20 T
Kategorie
Typ Assay-Kit
Specific against other
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Alias Null
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Organelle extraction and staining
Manufacturer - Applications
Abbkine ExKine Nuclei Extraction Kit (High Purity) is designed for the preparation of pure nuclei and fragile nuclei from mammalian cultured cells and solid tissues. Nuclei are collected by ultracentrifugation through a sucrose cushion and are quite clean and free of contaminating membranes and cytoplasmic components. The isolated nuclei can be preserved frozen several months in the included storage buffer.
Manufacturer - Category
Cell analysis
Manufacturer - Conjugate / Tag
Nuclei
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
Background
The preparation of an extract from nuclei is often the first step for many cell biology applications, such as in vitro apoptosis, examination of the transcriptional status of cells and a source of nuclear components (chromatin, genomic DNA, histones and nuclear RNA/RNP).
Description
Abbkine ExKine™ Nuclei Extraction Kit (High Purity) is designed for the preparation of pure nuclei and fragile nuclei.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
• Preparation of pure nuclei and fragile nuclei from mammalian cultured cells and solid tissues.
• Protocol conducts ultracentrifugation through a dense sucrose cushion to protect nuclei and strip away membranes and cytoplasmic contaminants.
Usage Notes
Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Kit components
• Lysis Buffer
• 1.8M Sucrose Cushion
• Sucrose Dilution
• Nuclei Storage Buffer
Product Assortment
WB, ELISA

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 20 T
Lieferbar: In stock
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