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ExKine™ Mitochondrion Extraction Kit (Cultured Cells)

ArtNr ABK-KTP4003-50T
Hersteller Abbkine Scientific
Menge 50 T
Quantity options 200 T 50 T
Kategorie
Typ Assay-Kit
Specific against other
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Alias Null
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Organelle extraction and staining
Manufacturer - Applications
Abbkine ExKine Mitochondrion Extraction Kit (Cultured Cells) enables rapid and crude isolation of of intact mitochondria from cultured mammalian cells. The kit offers two protocols for the separation of mitochondria from cytosolic components relying on differential centrifugation. First protocol utilizes a reagent-based method allowing multiple samples to be processed concurrently. The second protocol uses traditional Dounce homogenization and provides approximately two-fold more mitochondria.
Manufacturer - Category
Cell analysis
Manufacturer - Conjugate / Tag
Mitochondrion
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
Background
Mitochondria is a double-membrane-bound organelle found in most eukaryotic cells. The function of mitochondria is to provide cellular energy. Moreover, mitochondria are involved in other tasks, such as signaling, cellular differentiation, and cell death, as well as maintaining control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders, cardiacdysfunction, heart failure and autism. Mitochondria may play an important role in these cellular processes.
Description
Abbkine ExKine™ Mitochondrion Extraction Kit (Cultured Cells) enables isolation of of intact mitochondria from cultured mammalian cells.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
• Isolate intact mitochondria from 20 million cells in less than 1 hour.
• Process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method.
• Isolated mitochondria is compatible with almost any downstream application.
Usage Notes
Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Kit components
• Lysis Buffer A (5x)
• Lysis Buffer B
• Lysis Buffer C
• Storage Buffer
Product Assortment
WB, ELISA

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 T
Lieferbar: In stock
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