Recombinant Human FGF-2/bFGF Protein

< 1.0 EU/μg of the protein by LAL method.

ice pack

16.41 kDa

Recombinant Human FGF-2/bFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Pro143-Ser288) of human FGF2 (Accession #NP_001997.5).

Pro143-Ser288

No tag

Growth Factor, Cell Culture related

1. Measured by its binding ability in a functional ELISA. Immobilized Human FGF2 at 0. 5 μg/mL (100 μL/well) can bind Human GPC3 with a linear range of 7-20 ng/mL.|2. Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED₅₀ for this effect is typically 0. 635-2. 54 ng/mL, corresponding to a specific activity of 3. 94 × 10⁵~1. 57 × 10⁶ units/mg.|3. Recombinant Human VEGFA(40 ng/mL, Cat. RP01162) and bFGF(50 ng/mL) induce mesoderm cells to differentiate into hematopoietic stem and progenitor cells. After 4 days induction, pebbly-like CD43+ hematopoietic stem and progenitor cells appeared in the hematogenic endothelium.|4. The primary neural stem cells were cultured with 20 ng/mL bFGF and observed every 24 h. Results showed that the particle size of the suspended neural stem cells gradually increased.

16.41 kDa