SCBN cells

Disease:
Normal
Origin:
Morphologically the cells resemble epithelial cells; however, the cells do not contain keratin and definitive evidence of epithelial origin is lacking.
Species:
Homo sapiens, human
Tissue:
Colon
Morphology:
Morphologically the cells resemble epithelial cells; however, the cells do not contain keratin and definitive evidence of epithelial origin is lacking.
Properties:
Adherent
Cytogenic data:
The line is diploid and no consistent marker chromosomes were observed.
Patient:
21 weeks gestation fetus
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
Remove medium, and rinse with 0.05% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:2 to 1:3 is recommended. Renew medium once every 3 to 4 days depending on cell density.
Freezing Medium:
Complete growth medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 10, 11
D13S317: 11, 13
D16S539: 10, 11
D5S818:12, 13
D7S820: 11
THO1:7, 8
TPOX: 9, 10
vWA: 14, 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: CCD 841 CoN (AddexBio P0009023)

Cell Lines