Disease:
Normal
Origin:
Morphologically the cells resemble epithelial cells; however, the cells do not contain keratin and definitive evidence of epithelial origin is lacking.
Species:
Homo sapiens, human Tissue:
Colon Morphology:
Morphologically the cells resemble epithelial cells; however, the cells do not contain keratin and definitive evidence of epithelial origin is lacking. Properties:
Adherent Cytogenic data:
The line is diploid and no consistent marker chromosomes were observed.Patient:
21 weeks gestation fetus Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture:
Remove medium, and rinse with 0.05% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:2 to 1:3 is recommended. Renew medium once every 3 to 4 days depending on cell density. Freezing Medium:
Complete growth medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative DNA Profile (STR):
Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 13 D16S539: 10, 11 D5S818:12, 13 D7S820: 11 THO1:7, 8 TPOX: 9, 10 vWA: 14, 18 Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: CCD 841 CoN (AddexBio P0009023)