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Acute myeloid leukemia (AML) Origin: Established from the peripheral blood Species:Homo sapiens Tissue:Blood Properties:Suspension Cytogenic data:Hyperdiploid karyotype Patient:Male, 20 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 20% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Split saturated culture 1:2 to 1:3. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Established from the peripheral blood Species:Homo sapiens Tissue:Blood Properties:Suspension Cytogenic data:Hyperdiploid karyotype Patient:Male, 20 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 20% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Split saturated culture 1:2 to 1:3. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Homo sapiens Tissue:Blood Properties:Suspension Cytogenic data:Hyperdiploid karyotype Patient:Male, 20 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 20% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Split saturated culture 1:2 to 1:3. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Blood Properties:Suspension Cytogenic data:Hyperdiploid karyotype Patient:Male, 20 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 20% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Split saturated culture 1:2 to 1:3. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Suspension Cytogenic data:Hyperdiploid karyotype Patient:Male, 20 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 20% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Split saturated culture 1:2 to 1:3. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Hyperdiploid karyotype Patient:Male, 20 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 20% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Split saturated culture 1:2 to 1:3. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Male, 20 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 20% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Split saturated culture 1:2 to 1:3. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
AddexBio-formulated RPMI-1640 Medium (C0004-01) + 20% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Split saturated culture 1:2 to 1:3. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Split saturated culture 1:2 to 1:3. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Split saturated culture 1:2 to 1:3. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Mutations in MOLM-13 Cells
K562
LP-1
MOLM-13
RPMI-8226
U937
RAW 264.7
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