Disease:
Acute myeloid leukemia (AML) Origin:
Established from the peripheral blood Species:
Homo sapiens Tissue:
Blood Properties:
Suspension Cytogenic data:
Hyperdiploid karyotype Patient:
Male, 20 yrs of age Medium:
AddexBio-formulated RPMI-1640 Medium (C0004-01) + 20% FBS Subculture:
Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:
Split saturated culture 1:2 to 1:3. Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR):
Amelogenin: X, Y; CSF1PO: 10; D13S317: 10, 11; D16S539: 10, 11; D5S818: 10, 11; D7S820: 10, 12; THO1: 7; TPOX: 8; vWA: 16, 17 Protein Expression:
CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR - Mutations in MOLM-13 Cells
Related cells by Organ:
CCRF-CEM K562
LP-1
MOLM-13
RPMI-8226
U937
RAW 264.7