Raji cells

Hersteller	Addexbio
Kategorie	Oncology Metabolism Cholesterol & Lipid Metabolism Enzymes Other Fatty Acid Enzymes Cell Lines & Cell Culture Cell Lines By Organ Skin Cancer Lymphatic System Cancer
Typ	Tumour Cells
Specific against	Human
Menge	One Frozen vial
ArtNr	C0003009
eClass 6.1	32160890
eClass 9.0	32160890
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Biosafety Level	I (The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1).
Cytogenic Data	human flat-moded hypotetraploid karyotype with 12% polyploidy - 89(80-91)<4n>XXYY, -4, -9, -12, -16, -18, -21, +3mar, add(4)(q35), add(6)(q27)x2, t(8; 14)(q24; q32), der(8)t(8; 14) (q24; q32)add(8)(?p12), del(9)(p23), der(14)t(8; 14)(q24; q32) - carries t(8; 14) associated with Burkitt lymphoma - closely resembles depositor's karyotype
DNA Profile	Amelogenin: X, Y; CSF1PO: 10, 12; D13S317: 13; D16S539: 8, 11; D5S818: 10, 13; D7S820: 10; THO1: 6, 7; TPOX: 8, 13; vWA: 16, 19
Disease	Burkitt's lymphoma
Freezing Medium	Complete culture medium supplemented with 5% (v/v) DMSO
Medium	RPMI1640 + 10% FBS
Morphology	Lymphoblast
Origin	Established from the left maxilla of a 12-year-old African boy with Burkitt lymphoma in 1963; first continuous human hematopoietic cell line
Pathogens	HIV: Negative, Hepatitis B: Negative, Hepatitis C: Negative
Patient	Male, Black, 11 yrs of age
Properties	Round single cells in suspension, also in clusters or clumps
Sterility	Bacteria: Negative, Yeast: Negative, Mycoplasma: Negative
Subculture	Optimal split ratio of saturated culture at 1:4 to 1:8 twice a week; seed out at 0.5 x 10^6 cells/ml; maximum of 1.5x10^6 viable cells/mL is considered saturated culture; 5% CO2; 37 C
Tissue	Lymphoblast
Storage condition	LN2
Manufacturers Category	Cell Lines

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Menge: One Frozen vial

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Lieferung vsl. bis 30.05.2024

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