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Raji cells

Raji cells

Manufacturer	Addexbio
Category	Oncology Metabolism Cholesterol & Lipid Metabolism Enzymes Other Fatty Acid Enzymes Cell Lines & Cell Culture Cell Lines By Organ Skin Cancer Lymphatic System Cancer
Type	Tumour Cells
Specific against	Human
Amount	One Frozen vial
Item no.	C0003009
eClass 6.1	32160890
eClass 9.0	32160890
Available
Biosafety Level	I (The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1).
Cytogenic Data	human flat-moded hypotetraploid karyotype with 12% polyploidy - 89(80-91)<4n>XXYY, -4, -9, -12, -16, -18, -21, +3mar, add(4)(q35), add(6)(q27)x2, t(8; 14)(q24; q32), der(8)t(8; 14) (q24; q32)add(8)(?p12), del(9)(p23), der(14)t(8; 14)(q24; q32) - carries t(8; 14) associated with Burkitt lymphoma - closely resembles depositor's karyotype
DNA Profile	Amelogenin: X, Y; CSF1PO: 10, 12; D13S317: 13; D16S539: 8, 11; D5S818: 10, 13; D7S820: 10; THO1: 6, 7; TPOX: 8, 13; vWA: 16, 19
Disease	Burkitt's lymphoma
Freezing Medium	Complete culture medium supplemented with 5% (v/v) DMSO
Medium	RPMI1640 + 10% FBS
Morphology	Lymphoblast
Origin	Established from the left maxilla of a 12-year-old African boy with Burkitt lymphoma in 1963; first continuous human hematopoietic cell line
Pathogens	HIV: Negative, Hepatitis B: Negative, Hepatitis C: Negative
Patient	Male, Black, 11 yrs of age
Properties	Round single cells in suspension, also in clusters or clumps
Sterility	Bacteria: Negative, Yeast: Negative, Mycoplasma: Negative
Subculture	Optimal split ratio of saturated culture at 1:4 to 1:8 twice a week; seed out at 0.5 x 10^6 cells/ml; maximum of 1.5x10^6 viable cells/mL is considered saturated culture; 5% CO2; 37 C
Tissue	Lymphoblast
Storage condition	LN2
Manufacturers Category	Cell Lines

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: One Frozen vial

available

Delivery expected until 5/30/2024