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Acute lymphoblastic leukemia; B cell precursor leukemia
Established from the bone marrow of a 32-year-old woman with acute lymphoblastic leukemia (ALL L2) in first relapse
Homo sapiens Tissue:Bone marrow Morphology:Lymphoblast Properties: Suspension; single, relatively small, round cells Cytogenic data: human hyperdiploid karyotype - 47/48< 2n> X/XX, +8, +18, t(4; 11)(q21; q23), i(7q) - sideline without +8/18 - carries MLL-AFF1 (MLL-AF4) fusion gene - resembles published karyotype Patient: Female, Caucasian, 32 yrs of age Medium: RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 X 10^6 viable cells/mL. Split saturated culture 1:3 to 1:4 every 3-4 days; Maximal density of about 4.0 x 10^6 cells/ml; 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
Bone marrow Morphology:Lymphoblast Properties: Suspension; single, relatively small, round cells Cytogenic data: human hyperdiploid karyotype - 47/48< 2n> X/XX, +8, +18, t(4; 11)(q21; q23), i(7q) - sideline without +8/18 - carries MLL-AFF1 (MLL-AF4) fusion gene - resembles published karyotype Patient: Female, Caucasian, 32 yrs of age Medium: RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 X 10^6 viable cells/mL. Split saturated culture 1:3 to 1:4 every 3-4 days; Maximal density of about 4.0 x 10^6 cells/ml; 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
Lymphoblast Properties: Suspension; single, relatively small, round cells Cytogenic data: human hyperdiploid karyotype - 47/48< 2n> X/XX, +8, +18, t(4; 11)(q21; q23), i(7q) - sideline without +8/18 - carries MLL-AFF1 (MLL-AF4) fusion gene - resembles published karyotype Patient: Female, Caucasian, 32 yrs of age Medium: RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 X 10^6 viable cells/mL. Split saturated culture 1:3 to 1:4 every 3-4 days; Maximal density of about 4.0 x 10^6 cells/ml; 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
Suspension; single, relatively small, round cells Cytogenic data: human hyperdiploid karyotype - 47/48< 2n> X/XX, +8, +18, t(4; 11)(q21; q23), i(7q) - sideline without +8/18 - carries MLL-AFF1 (MLL-AF4) fusion gene - resembles published karyotype Patient: Female, Caucasian, 32 yrs of age Medium: RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 X 10^6 viable cells/mL. Split saturated culture 1:3 to 1:4 every 3-4 days; Maximal density of about 4.0 x 10^6 cells/ml; 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
human hyperdiploid karyotype - 47/48< 2n> X/XX, +8, +18, t(4; 11)(q21; q23), i(7q) - sideline without +8/18 - carries MLL-AFF1 (MLL-AF4) fusion gene - resembles published karyotype Patient: Female, Caucasian, 32 yrs of age Medium: RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 X 10^6 viable cells/mL. Split saturated culture 1:3 to 1:4 every 3-4 days; Maximal density of about 4.0 x 10^6 cells/ml; 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
Female, Caucasian, 32 yrs of age Medium: RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 X 10^6 viable cells/mL. Split saturated culture 1:3 to 1:4 every 3-4 days; Maximal density of about 4.0 x 10^6 cells/ml; 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 X 10^6 viable cells/mL. Split saturated culture 1:3 to 1:4 every 3-4 days; Maximal density of about 4.0 x 10^6 cells/ml; 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 X 10^6 viable cells/mL. Split saturated culture 1:3 to 1:4 every 3-4 days; Maximal density of about 4.0 x 10^6 cells/ml; 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
Complete culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17
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