RS4, 11 cells

Disease:
Acute lymphoblastic leukemia; B cell precursor leukemia
Origin:
Established from the bone marrow of a 32-year-old woman with acute lymphoblastic leukemia (ALL L2) in first relapse
Species:
Homo sapiens
Tissue:
Bone marrow
Morphology:
Lymphoblast
Properties:
Suspension; single, relatively small, round cells
Cytogenic data:
human hyperdiploid karyotype - 47/48< 2n> X/XX, +8, +18, t(4; 11)(q21; q23), i(7q) - sideline without +8/18 - carries MLL-AFF1 (MLL-AF4) fusion gene - resembles published karyotype
Patient:
Female, Caucasian, 32 yrs of age
Medium:
RPMI-1640 Medium + 10% FBS
Subculture:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 X 10^6 viable cells/mL. Split saturated culture 1:3 to 1:4 every 3-4 days; Maximal density of about 4.0 x 10^6 cells/ml; 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR): Amelogenin: X; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 8, 12; THO1: 6, 9.3; TPOX: 8; vWA: 14, 17

Cell Lines