BJAB cells

Disease:

Burkitt lymphoma

Origin:

Established from the inguinal tumor of an EBV-negative 5-year-old African girl with Burkitt lymphoma at the preterminal stage.

Species:

Homo sapiens

Tissue:

Peripheral blood

Morphology:

Round to polymorphic cells

Properties:

Cells growing in suspension, singly and in clumps

Cytogenic data:

human hypodiploid karyotype; 44(40-46)< 2n> XX, -10, -21, der(6)t(6; 10)(p22; p12), der(7)t(1; 7)(p36; p22), t(11; 17)(q24; q24), der(11)t(11; 17)(q24; q24)t(11; 6)(p15; q11)t(6; 5)(q22; ?), ider(13)(q10)t(3; 13)(q26; q14), der(13)t(10; 13)(q11; p11)dup(13)(q11q32)t(13; 21)(q32; q22), der(21)t(13; 21); no IG/MYC translocation detected

Patient:

African female, 5 yrs of age

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS

Subculture:

Seed cells at approximately 6 x 10⁵ cells/ml; optimal split ratio 1:2 every 2-3 days; culture flask should be kept horizontal. Although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Culture at 5% CO₂; 37C

Freezing Medium:

Complete culture medium supplemented with 10% (v/v) DMSO