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Burkitt lymphoma
Established from the inguinal tumor of an EBV-negative 5-year-old African girl with Burkitt lymphoma at the preterminal stage.
Homo sapiens Tissue:Peripheral blood Morphology:Round to polymorphic cells Properties: Cells growing in suspension, singly and in clumps Cytogenic data:human hypodiploid karyotype; 44(40-46)< 2n> XX, -10, -21, der(6)t(6; 10)(p22; p12), der(7)t(1; 7)(p36; p22), t(11; 17)(q24; q24), der(11)t(11; 17)(q24; q24)t(11; 6)(p15; q11)t(6; 5)(q22; ?), ider(13)(q10)t(3; 13)(q26; q14), der(13)t(10; 13)(q11; p11)dup(13)(q11q32)t(13; 21)(q32; q22), der(21)t(13; 21); no IG/MYC translocation detected Patient: African female, 5 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS Subculture:Seed cells at approximately 6 x 105 cells/ml; optimal split ratio 1:2 every 2-3 days; culture flask should be kept horizontal. Although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Peripheral blood Morphology:Round to polymorphic cells Properties: Cells growing in suspension, singly and in clumps Cytogenic data:human hypodiploid karyotype; 44(40-46)< 2n> XX, -10, -21, der(6)t(6; 10)(p22; p12), der(7)t(1; 7)(p36; p22), t(11; 17)(q24; q24), der(11)t(11; 17)(q24; q24)t(11; 6)(p15; q11)t(6; 5)(q22; ?), ider(13)(q10)t(3; 13)(q26; q14), der(13)t(10; 13)(q11; p11)dup(13)(q11q32)t(13; 21)(q32; q22), der(21)t(13; 21); no IG/MYC translocation detected Patient: African female, 5 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS Subculture:Seed cells at approximately 6 x 105 cells/ml; optimal split ratio 1:2 every 2-3 days; culture flask should be kept horizontal. Although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Round to polymorphic cells Properties: Cells growing in suspension, singly and in clumps Cytogenic data:human hypodiploid karyotype; 44(40-46)< 2n> XX, -10, -21, der(6)t(6; 10)(p22; p12), der(7)t(1; 7)(p36; p22), t(11; 17)(q24; q24), der(11)t(11; 17)(q24; q24)t(11; 6)(p15; q11)t(6; 5)(q22; ?), ider(13)(q10)t(3; 13)(q26; q14), der(13)t(10; 13)(q11; p11)dup(13)(q11q32)t(13; 21)(q32; q22), der(21)t(13; 21); no IG/MYC translocation detected Patient: African female, 5 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS Subculture:Seed cells at approximately 6 x 105 cells/ml; optimal split ratio 1:2 every 2-3 days; culture flask should be kept horizontal. Although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Cells growing in suspension, singly and in clumps Cytogenic data:human hypodiploid karyotype; 44(40-46)< 2n> XX, -10, -21, der(6)t(6; 10)(p22; p12), der(7)t(1; 7)(p36; p22), t(11; 17)(q24; q24), der(11)t(11; 17)(q24; q24)t(11; 6)(p15; q11)t(6; 5)(q22; ?), ider(13)(q10)t(3; 13)(q26; q14), der(13)t(10; 13)(q11; p11)dup(13)(q11q32)t(13; 21)(q32; q22), der(21)t(13; 21); no IG/MYC translocation detected Patient: African female, 5 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS Subculture:Seed cells at approximately 6 x 105 cells/ml; optimal split ratio 1:2 every 2-3 days; culture flask should be kept horizontal. Although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
human hypodiploid karyotype; 44(40-46)< 2n> XX, -10, -21, der(6)t(6; 10)(p22; p12), der(7)t(1; 7)(p36; p22), t(11; 17)(q24; q24), der(11)t(11; 17)(q24; q24)t(11; 6)(p15; q11)t(6; 5)(q22; ?), ider(13)(q10)t(3; 13)(q26; q14), der(13)t(10; 13)(q11; p11)dup(13)(q11q32)t(13; 21)(q32; q22), der(21)t(13; 21); no IG/MYC translocation detected Patient: African female, 5 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS Subculture:Seed cells at approximately 6 x 105 cells/ml; optimal split ratio 1:2 every 2-3 days; culture flask should be kept horizontal. Although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
African female, 5 yrs of age Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS Subculture:Seed cells at approximately 6 x 105 cells/ml; optimal split ratio 1:2 every 2-3 days; culture flask should be kept horizontal. Although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS Subculture:Seed cells at approximately 6 x 105 cells/ml; optimal split ratio 1:2 every 2-3 days; culture flask should be kept horizontal. Although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Seed cells at approximately 6 x 105 cells/ml; optimal split ratio 1:2 every 2-3 days; culture flask should be kept horizontal. Although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Complete culture medium supplemented with 10% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Hepatitis B: Negative Hepatitis C: Negative
Hepatitis C: Negative
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