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Biphenotypic B myelomonocytic leukemia
This cell line was established from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia.
Homo sapiens, human Tissue: Peripheral blood Morphology: Suspension Properties: Macrophagic Cytogenic data: 48, XY, t(4; 11)(q21; q23), +8, +19 Patient: Male, 10 years of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS Subculture: Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 2x10^5 viable cells/mL and maintain between 1x10^5 and 1x10^6 cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
Peripheral blood Morphology: Suspension Properties: Macrophagic Cytogenic data: 48, XY, t(4; 11)(q21; q23), +8, +19 Patient: Male, 10 years of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS Subculture: Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 2x10^5 viable cells/mL and maintain between 1x10^5 and 1x10^6 cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
Suspension Properties: Macrophagic Cytogenic data: 48, XY, t(4; 11)(q21; q23), +8, +19 Patient: Male, 10 years of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS Subculture: Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 2x10^5 viable cells/mL and maintain between 1x10^5 and 1x10^6 cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
Macrophagic Cytogenic data: 48, XY, t(4; 11)(q21; q23), +8, +19 Patient: Male, 10 years of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS Subculture: Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 2x10^5 viable cells/mL and maintain between 1x10^5 and 1x10^6 cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
48, XY, t(4; 11)(q21; q23), +8, +19 Patient: Male, 10 years of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS Subculture: Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 2x10^5 viable cells/mL and maintain between 1x10^5 and 1x10^6 cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
Male, 10 years of age Medium: AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS Subculture: Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 2x10^5 viable cells/mL and maintain between 1x10^5 and 1x10^6 cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS Subculture: Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 2x10^5 viable cells/mL and maintain between 1x10^5 and 1x10^6 cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 2x10^5 viable cells/mL and maintain between 1x10^5 and 1x10^6 cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
Complete culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
Amelogenin: X, Y CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
CSF1PO: 10, 12 D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
D13S317: 13 D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
D16S539: 11, 12 D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
D5S818: 11, 12 D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
D7S820: 8, 9 THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
THO1: 8, 9.3 TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
TPOX: 8, 11 vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
vWA: 14, 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
If use of this culture results in a scientific publication, it should be cited in the publication as: MV-4-11 (AddexBio C0003025)
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