Mino cells

Disease:

Mantle Cell Lymphoma

Origin:

The cells are large, growing singly and in small clumps in vitro. The immunophenotype by flow cytometry is compatible with MCL. Western blots show expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein is predominantly phosphorylated. There is expression of tumor suppressor gene products including p53, p16 (INK4a), and p21(WAF1).

Species:

Homo sapiens

Tissue:

peripheral blood/Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)

Morphology:

Lymphoblast

Properties:

Suspension

Cytogenic data:

Modal chromosome number: 74. This is a hypertriploid male cell line, del(6)(q16), t(8; 11)(q22; q23), add(11)(q25), add(13)(q34), add(14)(q32). Most cells contain the t(11; 14) derivative chromosome, detected by FISH with whole chromosome paint probes.

Patient:

Male, 64 years of age, Caucasian

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium + 15% FBS

Subculture:

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 6 X 10^4 cells/mL and maintain between 1 X 10^5 and 1 X 10^6 cells/mL. Do not allow the cell concentration to reach 1.5x10^6 cells/mL. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO