Disease:
Mantle Cell Lymphoma
Origin:
The cells are large, growing singly and in small clumps in vitro. The immunophenotype by flow cytometry is compatible with MCL. Western blots show expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein is predominantly phosphorylated. There is expression of tumor suppressor gene products including p53, p16 (INK4a), and p21(WAF1).
Species:
Homo sapiens Tissue:
peripheral blood/Mantle cell lymphoma (B cell non-Hodgkin's lymphoma) Morphology:
Lymphoblast Properties:
Suspension Cytogenic data:
Modal chromosome number: 74. This is a hypertriploid male cell line, del(6)(q16), t(8; 11)(q22; q23), add(11)(q25), add(13)(q34), add(14)(q32). Most cells contain the t(11; 14) derivative chromosome, detected by FISH with whole chromosome paint probes. Patient:
Male, 64 years of age, Caucasian Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium + 15% FBS Subculture:
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 6 X 10^4 cells/mL and maintain between 1 X 10^5 and 1 X 10^6 cells/mL. Do not allow the cell concentration to reach 1.5x10^6 cells/mL. Culture at 5% CO2, 37C. Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR):
Amelogenin: X, Y CSF1PO: 9, 11 D13S317: 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 10, 11 THO1: 9.3 TPOX: 8, 11 vWA: 14, 17 Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: Mino (AddexBio C0003033)