SK-N-SH cells

Disease:
Neuroblastoma
Origin:
Brain; derived from metastatic site: bone marrow. The SK-N-SH line differs from SK-N-MC in that it exhibits a longer doubling time and higher levels of dopamine - beta - hydroxylase.
Species:
Homo sapiens
Tissue:
Brain
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
2n = 46, modal no. 47
Patient:
Female, Caucasian, 4 years of age
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
When 80% confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; seed at 3x10^4 cells/cm2, feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8, 13
D5S818: 12
D7S820: 7, 10
THO1: 7, 10
TPOX: 8, 11
vWA: 14, 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: SK-N-SH (AddexBio C0005006)

Cell Lines