ZR-75-1 cells

Disease:
Ductal Carcinoma
Origin:
This cell line was derived from metastatic site: ascites.
Species:
Homo sapiens
Tissue:
Mammary gland/breast/duct
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
This human cell line has a hypertriploid chromosome number. The modal chromosome number was 72 occurring in 26% of the cells and the rate of higher ploidies was at 1.2%. Eighteen markers were common.They are: t(1q, ?), M2, M3, del(3) (p21), der(5)t(5; ?) (q35; ?), del(6) (q21), t(11q14q), t(11; 11) (p15; q11), der(14)t(2; 14) (q21; q32), t(17q, ?), M13, M14, M15, M16, M17, der(8)t(8; ?), t(9p, ?) and 19pt. Of these M13 was paired. Normal N14 was not found. N6 was single copied, X had 3 copies and N18 had 4 copies in each cell.
Patient:
Female, 63 yrs of age, Caucasian
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
Subculture:
Subculture at 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X, X
CSF1PO: 10, 11
D13S317: 9
D16S539: 11
D5S818: 13
D7S820: 10, 11
THO1: 7, 9.3
TPOX: 8
vWA: 16, 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: ZR-75-1 (AddexBio C0006011)
Related cells by Organ: 4T1
AU-565
BT-20
BT-474
BT-483
BT-549
JIMT-1
MCF-7
SK-BR-3
T47D
ZR-75-1

Cell Lines