ZR-75-1 cells

Disease:

Ductal Carcinoma

Origin:

This cell line was derived from metastatic site: ascites.

Species:

Homo sapiens

Tissue:

Mammary gland/breast/duct

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

This human cell line has a hypertriploid chromosome number. The modal chromosome number was 72 occurring in 26% of the cells and the rate of higher ploidies was at 1.2%. Eighteen markers were common.They are: t(1q, ?), M2, M3, del(3) (p21), der(5)t(5; ?) (q35; ?), del(6) (q21), t(11q14q), t(11; 11) (p15; q11), der(14)t(2; 14) (q21; q32), t(17q, ?), M13, M14, M15, M16, M17, der(8)t(8; ?), t(9p, ?) and 19pt. Of these M13 was paired. Normal N14 was not found. N6 was single copied, X had 3 copies and N18 had 4 copies in each cell.

Patient:

Female, 63 yrs of age, Caucasian

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS

Subculture:

Subculture at 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO