BT-474 cells

Disease:
Ductal Carcinoma
Origin:
This cell line was isolated from a solid, invasive ductal carcinoma of the breast.
Species:
Homo sapiens
Tissue:
Mammary gland/breast/duct
Morphology:
Epithelial
Properties:
Adherent, patchy
Cytogenic data:
The cell line is aneuploid human female (XO usually), with most chromosome counts in the hypertetraploid range. Several chromosomes (N11, N13, and N22) are absent, and others are clearly under-represented (N9, N14, and N15) with respect to the other normal chromosomes. Chromosome N7 tends towards over-representation in several karyotypes. Some of the missing normal chromosomes are represented by their involvement in the nine stable marker chromosomes: der(14)t(14; ?)(q32, ?), unknown, iso(13q), der(6)t(6; 7)(q21; q21), der(11)t(11; ?)(14; ?), del(11)(p11), unknown, unknown, der(2)t(2; ?)(p21; ?).
Patient:
Female, 60 yrs of age, Caucasian
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS + 10 ug/mL human insulin
Subculture:
Subculture at 1:3; feed cells 2 to 3 times weekly. Culture at 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X, X
CSF1PO: 10, 11
D13S317: 11
D16S539: 9, 11
D5S818: 11, 13
D7S820: 9, 12
THO1: 7
TPOX: 8
vWA: 15, 16
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: BT-474 (AddexBio C0006012)
Related cells by Organ: 4T1
AU-565
BT-20
BT-474
BT-483
BT-549
JIMT-1
MCF-7
SK-BR-3
T47D
ZR-75-1

Cell Lines