BT-474 cells

Disease:

Ductal Carcinoma

Origin:

This cell line was isolated from a solid, invasive ductal carcinoma of the breast.

Species:

Homo sapiens

Tissue:

Mammary gland/breast/duct

Morphology:

Epithelial

Properties:

Adherent, patchy

Cytogenic data:

The cell line is aneuploid human female (XO usually), with most chromosome counts in the hypertetraploid range. Several chromosomes (N11, N13, and N22) are absent, and others are clearly under-represented (N9, N14, and N15) with respect to the other normal chromosomes. Chromosome N7 tends towards over-representation in several karyotypes. Some of the missing normal chromosomes are represented by their involvement in the nine stable marker chromosomes: der(14)t(14; ?)(q32, ?), unknown, iso(13q), der(6)t(6; 7)(q21; q21), der(11)t(11; ?)(14; ?), del(11)(p11), unknown, unknown, der(2)t(2; ?)(p21; ?).

Patient:

Female, 60 yrs of age, Caucasian

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS + 10 ug/mL human insulin

Subculture:

Subculture at 1:3; feed cells 2 to 3 times weekly. Culture at 5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO