Disease:
Colorectal carcinoma Origin:
Established from the primary colon carcinoma Species:
Homo sapiens Tissue:
Colon Morphology:
Epithelial Properties:
Adherent Cytogenic data:
Tumorigenic; the stemline chromosome number is near diploid Patient:
Male, adult Medium:
AddexBio-Formulated DMEM (C0003-01) + 1X MEM Non-Essential Amino Acids (NEAA)(Thermo 11140050) + 10% FBS or McCoy's 5a Medium + 10% FBS Subculture:
1:3 to 1:8 using using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Isoenzymes:
AK-1, 1; ES-D, 1-2; G6PD, B; GLO-I, 1; PGM1, 1; PGM3, 1 DNA Profile (STR):
Amelogenin: X, Y; CSF1PO: 7, 10; D13S317: 10, 12; D16S539: 11, 13; D5S818: 10, 11; D7S820: 11, 12; THO1: 8, 9; TPOX: 8, 9; vWA: 17, 22 Mutations in HCT116 Cells
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: HCT-116 (AddexBio C0009005) Related cells by Organ:
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