HCT-116 cells

Disease:
Colorectal carcinoma
Origin:
Established from the primary colon carcinoma
Species:
Homo sapiens
Tissue:
Colon
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Tumorigenic; the stemline chromosome number is near diploid
Patient:
Male, adult
Medium:
AddexBio-Formulated DMEM (C0003-01) + 1X MEM Non-Essential Amino Acids (NEAA)(Thermo 11140050) + 10% FBS or McCoy's 5a Medium + 10% FBS
Subculture:
1:3 to 1:8 using using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Isoenzymes: AK-1, 1; ES-D, 1-2; G6PD, B; GLO-I, 1; PGM1, 1; PGM3, 1
DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 7, 10; D13S317: 10, 12; D16S539: 11, 13; D5S818: 10, 11; D7S820: 11, 12; THO1: 8, 9; TPOX: 8, 9; vWA: 17, 22 Mutations in HCT116 Cells
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: HCT-116 (AddexBio C0009005)
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Cell Lines