Disease:
Colorectal adenocarcinoma Origin:
Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon Species:
Homo sapiens Tissue:
Colon Morphology:
Epithelial Properties:
Adherent Cytogenic data:
Tumorigenic, Modal no. 45 (2n=46) Patient:
Female, Caucasian, 58 yrs of age Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture:
1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression:
HLA A2, B13, B50, IL6, IL10, mucin Isoenzymes:
ADA, 1; ES-D, 1; G6PD, B; PEP-D, 1; PGD, A; PGM1, 1; PGM3, 2 DNA Profile (STR):
Amelogenin: X ; CSF1PO: 10, 13; D13S317: 10; D16S539: 11, 13; D5S818: 10, 15; D7S820: 11, 9.3; THO1: 6, 7; TPOX: 8, 9; vWA: 15, 18 Mutations in LS-180 Cells
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: LS-180 (AddexBio C0009006) Related cells by Organ:
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