LS-180 cells

Disease:
Colorectal adenocarcinoma
Origin:
Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon
Species:
Homo sapiens
Tissue:
Colon
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Tumorigenic, Modal no. 45 (2n=46)
Patient:
Female, Caucasian, 58 yrs of age
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level: I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Protein Expression: HLA A2, B13, B50, IL6, IL10, mucin
Isoenzymes: ADA, 1; ES-D, 1; G6PD, B; PEP-D, 1; PGD, A; PGM1, 1; PGM3, 2
DNA Profile (STR): Amelogenin: X ; CSF1PO: 10, 13; D13S317: 10; D16S539: 11, 13; D5S818: 10, 15; D7S820: 11, 9.3; THO1: 6, 7; TPOX: 8, 9; vWA: 15, 18 Mutations in LS-180 Cells
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: LS-180 (AddexBio C0009006)
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Cell Lines