COLO 205 cells

Disease:
Dukes' type D, colorectal adenocarcinoma
Origin:
This line was isolated in 1975 from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen.
Species:
Homo sapiens
Tissue:
Colon; metastatic site: ascites
Morphology:
Epithelial; round and refractile cells in suspension. Some cuboidal cells in the monolayer. COLO 205 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 205 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer.
Properties:
Mixed, adherent and suspension
Cytogenic data:
Hypertriploid modal no 71-78
Patient:
70 year old Caucasian, male
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
Subculture:
Subculture at 1:6; feed cells 3 to 4 times weekly. Culture at 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 11, 12
D13S317: 10, 12
D16S539: 12, 13
D5S818: 10, 13
D7S820: 9, 10
THO1: 8, 9
TPOX: 11
vWA: 15
If use of this culture results in a scientific publication, it should be cited in the publication as: COLO 205 (AddexBio C0009018)
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Cell Lines