COLO 205 cells

Disease:

Dukes' type D, colorectal adenocarcinoma

Origin:

This line was isolated in 1975 from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen.

Species:

Homo sapiens

Tissue:

Colon; metastatic site: ascites

Morphology:

Epithelial; round and refractile cells in suspension. Some cuboidal cells in the monolayer. COLO 205 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 205 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer.

Properties:

Mixed, adherent and suspension

Cytogenic data:

Hypertriploid modal no 71-78

Patient:

70 year old Caucasian, male

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS

Subculture:

Subculture at 1:6; feed cells 3 to 4 times weekly. Culture at 5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO