SNU-182 cells

Disease:

Carcinoma, hepatocellular; grade III/IV

Origin:

SNU-182 was derived in 1989 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Asian patient prior to cytotoxic therapy.

Species:

Homo sapiens, human

Tissue:

Liver

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

aneuploid; modal number = 70

Patient:

Male, 24 years of age, Asian

Medium:

AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS

Subculture:

Split sub-confluent cultures (70-80%) at a subcultivation ratio of 1:3 to 1:6 is recommended. Subculture at higher cell density is recommended. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 7.5% (v/v) DMSO