SNU-182 cells

Disease:
Carcinoma, hepatocellular; grade III/IV
Origin:
SNU-182 was derived in 1989 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Asian patient prior to cytotoxic therapy.
Species:
Homo sapiens, human
Tissue:
Liver
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
aneuploid; modal number = 70
Patient:
Male, 24 years of age, Asian
Medium:
AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS
Subculture:
Split sub-confluent cultures (70-80%) at a subcultivation ratio of 1:3 to 1:6 is recommended. Subculture at higher cell density is recommended. Culture at 5% CO2, 37C.
Freezing Medium:
Complete culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
II
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 10, 12
D13S317: 11, 14
D16S539: 11, 13
D5S818: 11, 12
D7S820: 11
THO1: 9
TPOX: 8, 11
vWA: 17
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: SNU-182 (AddexBio C0015003)

Cell Lines