H23 cells

Disease:
Adenocarcinoma; non-small cell lung cancer
Origin:
Established from a lung cancer obtained from a patient prior to therapy.
Species:
Homo sapiens
Tissue:
Lung
Morphology:
Epithelial
Properties:
Adherent
Other information:
The cells carry the K-ras 12 mutation, and there is a mutation in codon 246 (ATC -> ATG, isoleucine -> methionine) of the p53 gene.
Express C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs.
Express heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR).
Exhibits a high degree of c-myc DNA amplification (20-fold) but no detectable amplification of c-myc RNA.
Positive for keratins 5+8 and 18 and vimentin but are negative for neurofilament.
L-dopa decarboxylase-negative.
Colony forming efficiency of 9.7% in soft agarose.
Patient:
Male, Black, 51 years of age
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
Doubling time:
38 hours
Subculture:
A subcultivation ratio of 1:3 to 1:5 is recommended.5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR): Amelogenin: X; CSF1PO: 10; D13S317: 12; D16S539: 11; D5S818: 12, 13; D7S820: 9, 10; THO1: 6; TPOX: 8, 9; vWA: 16, 17
If use of this culture results in a scientific publication, it should be cited in the publication as: H23 (AddexBio C0016005)
Related cells by Organ: A427 cells
A549 cells
Calu3 cells
H1650 cells
H1703 cells
H1975 cells
H226 cells
H23 cells
H460 cells
H520 cells
HCC4006 cells
SW1573 cells
3D4/2 cells
BEAS-2B cells
MRC-5 cells
Mv 1 Lu cells
RLE-6TN cells

Cell Lines