H23 cells

Disease:

Adenocarcinoma; non-small cell lung cancer

Origin:

Established from a lung cancer obtained from a patient prior to therapy.

Species:

Homo sapiens

Tissue:

Lung

Morphology:

Epithelial

Properties:

Adherent

Other information:

The cells carry the K-ras 12 mutation, and there is a mutation in codon 246 (ATC -> ATG, isoleucine -> methionine) of the p53 gene.

Express C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs.

Express heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR).

Exhibits a high degree of c-myc DNA amplification (20-fold) but no detectable amplification of c-myc RNA.

Positive for keratins 5+8 and 18 and vimentin but are negative for neurofilament.

L-dopa decarboxylase-negative.

Colony forming efficiency of 9.7% in soft agarose.

Patient:

Male, Black, 51 years of age

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS

Doubling time:

38 hours

Subculture:

A subcultivation ratio of 1:3 to 1:5 is recommended.5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO