H226 cells

Disease:
Squamous cell carcinoma; mesothelioma
Origin:
This cell line was derived from metastatic site: pleural effusion in March of 1980 from a patient with squamous cell carcinoma of the lung
Species:
Homo sapiens
Tissue:
Lung, Pleural effusion
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
modal number = 47; range = 32-88; del(p25)
Patient:
Male
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
Subculture:
When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 10, 11
D13S317: 13, 14
D16S539: 9, 12
D5S818: 11, 12
D7S820: 8, 10
THO1: 8, 9.3
TPOX: 8, 11
vWA: 17
If use of this culture results in a scientific publication, it should be cited in the publication as: H226 (AddexBio C0016011)
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Cell Lines